— CHAPTER MASTERY · CLASS 12

Biotechnology – Principles and Processes Important Questions.

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Key Concepts in Biotechnology – Principles and Processes

Tools of recombinant DNA technology: restriction enzymes (REases), cloning vectors (pBR322, plasmids, phage, BAC, YAC)Gel electrophoresis and Southern blottingPCR: steps (denaturation, annealing, extension), Taq polymerase, applicationsCloning and expression of recombinant protein: competent cells, transformation, selection (antibiotic resistance, blue-white)Upstream and downstream processing of biotech products

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